Journal: Scientific Reports

Article Title: Intraoperative detection of circulating tumor cells in pulmonary venous blood during metastasectomy for colorectal lung metastases

doi: 10.1038/s41598-018-26410-8

Figure Lengend Snippet: Clinical, surgical and follow-up data.

Article Snippet: For CTC-Isolation each sample was filtered through CellSieve microfilters using the CellSieve CTC Isolation Kit (Creatv MicroTech, Inc., Potomac, MD, USA) and a programmable syringe pump (LEGATO 110; KD Scientific Inc., Holliston, MA, USA) following manufacturer’s instructions.

Techniques:

Journal: Cancers

Article Title: Automated Capture and Analysis of Circulating Tumor Cells in Pediatric, Adolescent and Young Adult Patients with Central Nervous System Tumors

doi: 10.3390/cancers15153853

Figure Lengend Snippet: Circulating Tumor Cell (CTC) Isolation Workflow. Freshly collected (<24 h) patient peripheral blood is subjected to gradient centrifugation (1) to isolate peripheral blood mononuclear cells (PBMCs) (2). PBMCs are separated and collected into a 200 µL suspension (3) which is loaded onto the Abnova CytoQuest CTC isolation platform (4). After automated processing, CTCs are bound to the cell surface vimentin (CSV)-coated CTC chip (5). Cells captured on the chip are then stained (CSV, CD45, DAPI) and imaged.

Article Snippet: In this study, we used a CSV-coated cell capture chip, the Abnova CytoQuest automated CTC isolation system, to boost the CTC capture from pediatric patients with CNS tumors.

Techniques: Isolation, Gradient Centrifugation, Suspension, Staining

Journal: Clinical cancer research : an official journal of the American Association for Cancer Research

Article Title: Mutation analysis of cell-free DNA and single circulating tumor cells in metastatic breast cancer patients with high CTC counts

doi: 10.1158/1078-0432.CCR-16-0825

Figure Lengend Snippet: 112 patients with metastatic breast cancer were recruited to the study. Blood was collected and analysed for CTC count and total cfDNA levels in all 112 patients. In 5 patients with high CTC count individual CTCS were isolated by DEPArray and subjected to targeted NGS in comparison with matched cfDNA and primary tumour tissue where available.

Article Snippet: CTC isolation by DEPArray, QC to identify those samples with a genomic integrity index (GII) of 3 or 4 suitable for sequencing ( 20 ) and Ampli1 TM whole genome amplification were carried out as a service by Silicon Biosystems.

Techniques: Isolation, Comparison

Journal: British Journal of Cancer

Article Title: Detection and prognostic role of heterogeneous populations of melanoma circulating tumour cells

doi: 10.1038/s41416-020-0750-9

Figure Lengend Snippet: a Comparison of the Parsortix (Angle plc), ClearCell FX1 (Biolidics) (LP: low purity and HP: high purity programmes) and the Slanted spiral microfluidics platforms, on their recovery rates of the melanoma cell lines A2058 or SK-MEL-5 (indicated as bars: left y-axis) and total WBC counts (indicated in green as mean ± SD: right y -axis). b WBC counts in CTC enriched samples, from 10 patients with metastatic melanoma, using the Parsortix (range: 965–12831) and ClearCell systems (LP mode, range: 4925–17732). c and d Expression levels of MLANA, TYR, MAGEA3 , ABCB5, and PAX3 assessed by RT-PCR in matched CTC fractions enriched by c Parsortix and d ClearCell platforms. Each heatmap squares represents the expression levels for each gene and a given sample, shown by reciprocal Ct values. The 18S rRNA gene was used as an endogenous control. The CTC counts obtained by multimarker immunostaining for each matched CTC fraction derived from the same patient are indicated. NA data not available.

Article Snippet: Using CTC fractions isolated by Parsortix (Angle plc), we investigated the molecular heterogeneity of CTCs isolated from 43 blood samples from metastatic melanoma patients.

Techniques: Comparison, Expressing, Reverse Transcription Polymerase Chain Reaction, Control, Immunostaining, Derivative Assay

Journal: British Journal of Cancer

Article Title: Detection and prognostic role of heterogeneous populations of melanoma circulating tumour cells

doi: 10.1038/s41416-020-0750-9

Figure Lengend Snippet: a Representative CTCs isolated from melanoma patients using Parsortix (Angle plc) or ClearCell FX1 (Biolidics). CTCs were identified through positive immunostaining for MCSP (Alexa Fluor® 647, purple) and intracellular -MEL (gp100, S100 and MLANA; Alexa Fluor® 488, green) markers. DAPI was used for nuclei staining and CD45 (PE, red) as a leucocytic marker. Immunofluorescence intensity of patients CTCs for b the cell surface marker MCSP and c the combination of the melanoma-specific intracellular markers (MEL staining). CTCs recovered with either device are indicated in different colours. The immunofluorescence intensity of A2058 cells spiked into healthy donors’ blood, recovered and detected using the same methods used for patient samples, were included as a reference and labelled as “spike". d Cell size distribution of melanoma CTCs. Merged images of the two largest CTCs observed were included. Graphs are represented as scatter plots with median and interquartiles shown as box and whiskers plots. Scale bars indicate 10 µm.

Article Snippet: Using CTC fractions isolated by Parsortix (Angle plc), we investigated the molecular heterogeneity of CTCs isolated from 43 blood samples from metastatic melanoma patients.

Techniques: Isolation, Immunostaining, Staining, Marker, Immunofluorescence